1987 – New York

The Tenth Histocompatibility Workshop

The Tenth Histocompatibility Workshop, organized by Bo Dupont and held in New York City in November of 1987, had a number of specific objectives, including the standardization of assays used to characterize HLA genes and their products at the molecular, genetic, biochemical and cellular level, and the development of a reference panel of B-lymphoblastoid cell lines for factors of the HLA system. The workshop was divided into eight components, including serology, biochemistry, cellular and DNA sections. The serology component was organized into 34 different Antigen Groups to study antigen clusters of similar specificity. A total of 362 laboratories participated in the workshop.  A large number of new HLA specificities were defined in this workshop by a variety of techniques: 62 new specificities were defined by serology; 94 alleles were defined by biochemistry (one-dimensional iso-electric focusing); and seven T-cell defined HLA-Dw specificities were defined using T cell clones. This workshop was the first to implement DNA-based HLA typing with the RFLP Southern blot methodology using a standard panel of cDNA probes for HLA class I and class II, with separate analysis of DRA, DRB, DQA, DQB, DPA and DPB. A total of 80 laboratories used RFLP to analyze the core workshop cell line panel as well as local samples. RFLP patterns were defined which corresponded with the serologically defined HLA class II alleles. Thus, DNA typing was established as a method for typing of HLA. Some changes in HLA nomenclature were made to accommodate the designation of HLA alleles, based on amino acid sequences deduced from gene sequences. The reference panel of 107 B-lymphoblastoid cell lines remains a reference resource for well-characterized HLA material to this day.

Reference: Dupont B, ed. Immunobiology of HLA. Histocompatibility Testing 1987, Vol. I, and Immunogenetics and Histocompatibility, Vol II. Springer-Verlag, New York, 1988.

 

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