Project: HLA Allele Catalogue

The HLA Allele Cataloguing Project

Project leaders: Faviel Gonzalez, Derek Middleton, Kazutoyo Osoegawa & Medhat Askar

Detailed project description:

In this project participants are invited to submit data of samples of HLA alleles that are novel, classified as rare or with no enough information in the Allele Frequency Net Database (AFND, http://www.allelefrequencies.net). The project has 2 components:

  1. Submission of data to AFND confirming alleles that fulfil the criteria set above. These data will also be shared with the CWD alleles working group to be considered in generating future CWD alleles are equivalent/alternate lists.
  2. Submitters of alleles not tested by NGS but otherwise meet the above criteria may be invited to submit DNA samples to be confirmed by NGS based HLA typing in the Baylor University Medical Center Transplant Immunology Lab in Dallas, TX, USA.

Milestones in years:

2019: Submission of sample profiles and other pertinent data and preliminary analysis

2020 (Jan – July): Testing of samples that need confirmatory NGS testing and further analysis

2020 (Aug – Dec): Re-analyze HLA genotypes using organizer defined IPD-IMGT database version

2021: Final analysis and preparation of final report for presenting during the workshop

Patient/sample description (if applicable, details, inclusion/exclusion criteria):

HLA typing results of samples deemed by submitter to meet the project criteria

Data required (number, type of data, inclusion/exclusion criteria):

DNA testing results

Fastq files of samples tested by NGS

Any pertinent information to the subject carrying the allele of interest such as ethnic background, haplotype association determined by a family study

No max or min number of samples required

Samples required (if applicable, number, type of samples, inclusion/exclusion criteria):

If needed 2 ug DNA of high quality suitable for. Long range PCR amplification.

Reagents/additional assays required:

Not applicable

Data infrastructure required:

A database that house the collected typing results and fastq files similar to the database provided by the Stanford group during the 17th IHIW.


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